使用德国耶拿SELMA移液工作站提取DNA.
2013-03-29 15:33:03 益择发布Semi-automated genomic DNA extraction
from Plant with CyBi®-SELMA 96/1000 μl
using NucleoSpin® 96 Plant II Kit
VanessaClouet,MaryseLodeandAngéliqueLesné,INRAUMR1349IGEPP,35653LeRheuFrance
Key words: CyBi®-SELMA,MACHEREY-NAGELNucleoSpin96PlantIIkit,PlantgenomicDNAextraction,
Highthroughput
Abstract
TheCyBi®-SELMA96/1000μlwasusedtoautomateMACHEREY-NAGELNucleoSpin96PlantIItechnologyfor
plantgenomicDNAextraction.Theuseofa96-wellpipettingheadenablesarapidautomatedprocedurewhile
maintainingthepurificationtechnology’shighlyreliableperformance.Theresultisgoodyields(1-8μgspecies
function)ofhighlypurifiedDNA(A260/280>1.8).
Introduction
Thisextractionmethodisbasedontheestablished
CTABandSDSlysismetods(1).Asplantsarevery
heterogenousandcontainmanydifferent
metaboliteslikepolyphenols,polysaccharides,or
acidiccomponents,NucleoSpin®PlantIIofferstwo
differentlysisproceduresforoptimalprocessingof
varioussamples.WeusedthePL1lysisBufferbased
ontheCTABlysismethod.Inaddition,thesilica
membraneintheNucleoSpin®PlantIIColumnsis
optimizedtoimproveDNAbinding,andNucleoSpin®
Filtersare
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